1JIX
T4 Phage BGT in Complex with Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.943, 71.454, 62.010 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qkj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.040 | 0.149 |
| Total number of observations | 304335 * | |
| Number of reflections | 49706 | |
| <I/σ(I)> | 10 | 6.5 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 10000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG4000 | 10-11 (%) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | pH7.5 |
| 3 | 1 | drop | UDP | 1 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 20-22 (%) |
