1JIQ
Crystal Structure of Human Autocrine Motility Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-26 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.770, 107.400, 270.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.900 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.19791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.305 |
| Number of reflections | 183404 | |
| <I/σ(I)> | 4.6 | 2.4 |
| Completeness [%] | 98.7 | 92.1 |
| Redundancy | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | cacodylate, sodium acetate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
