1JIH

Yeast DNA Polymerase ETA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	163
Detector technology	CCD
Collection date	2001-01-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.97958,0.97941,0.96482
Spacegroup name	P 41 21 2
Unit cell lengths	105.100, 105.100, 292.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.226 *
Rwork	0.215
R-free	0.24900 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.265
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.250	2.250
Rmerge	0.074	0.192 *
Total number of observations	1335912 *
Number of reflections	70064 *
<I/σ(I)>	17.8	17.8
Completeness [%]	94.7	74 *
Redundancy	19	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	4 *	PEG 20K, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	unknown *	6.5	4 *	PEG 20K, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG20000	6 (%)
2	1	1	ammonium acetate	600 (mM)