1JIH
Yeast DNA Polymerase ETA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 163 |
| Detector technology | CCD |
| Collection date | 2001-01-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958,0.97941,0.96482 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 105.100, 105.100, 292.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.226 * |
| Rwork | 0.215 |
| R-free | 0.24900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.074 | 0.192 * |
| Total number of observations | 1335912 * | |
| Number of reflections | 70064 * | |
| <I/σ(I)> | 17.8 | 17.8 |
| Completeness [%] | 94.7 | 74 * |
| Redundancy | 19 | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 4 * | PEG 20K, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
| 1 | unknown * | 6.5 | 4 * | PEG 20K, Ammonium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG20000 | 6 (%) | |
| 2 | 1 | 1 | ammonium acetate | 600 (mM) |
