1JIG
Dlp-2 from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-08 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 87.723, 87.723, 214.875 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 1.460 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.20500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HP-NAP |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 18.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.032 | 0.068 |
| Number of reflections | 105192 | 13823 * |
| <I/σ(I)> | 12.7 | 9 |
| Completeness [%] | 98.2 | 88.2 |
| Redundancy | 3.0 * | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 20 * | Citrete buffer, ammonuium acetate, MPD, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | MPD | 20 (%) |
