1JIE

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with metal-free bleomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-10-01
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.520, 83.420, 43.240
Unit cell angles	90.00, 104.88, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.161 *
R-free	0.21900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qto
RMSD bond length	0.010
RMSD bond angle	0.032 *
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	0.169
Number of reflections	19023
<I/σ(I)>	10.4	2.25
Completeness [%]	80.3	55.1
Redundancy	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	25 *	PEG4000, ammonium acetate, sodium cacodylate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	17.5 (%(w/v))
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH5.6
4	1	drop	sodium phosphate	10 (mM)