1JI6
CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-04-15 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.440, 131.810, 105.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.25300 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 20.060 * |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 8.000 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 * | 0.076 |
| Total number of observations | 91281 * | |
| Number of reflections | 30077 | |
| <I/σ(I)> | 10.3 | 6.8 |
| Completeness [%] | 72.2 | 72.2 |
| Redundancy | 6.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 | 37 * | Cody, V., (1992) Proteins Struct. Funct. Genet., 14. 324. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 4 (M) | ||
| 2 | 1 | drop | HEPES | 0.1 (M) | |
| 3 | 1 | drop | Lubrol PX | 0.5 (%(w/v)) | |
| 4 | 1 | reservoir | 0.6 (M) | ||
| 5 | 1 | reservoir | HEPES | 0.1 (M) | |
| 6 | 1 | reservoir | Lubrol PX | 0.5 (%(w/v)) |
