1JI5

Dlp-1 from bacillus anthracis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-12
Detector	MARRESEARCH
Wavelength(s)	1.2
Spacegroup name	H 3
Unit cell lengths	89.224, 89.224, 210.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.500
R-factor	0.226
Rwork	0.226
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HP-NAP model
RMSD bond length	0.009
RMSD bond angle	18.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.100	0.380
Number of reflections	20677	3107 *
<I/σ(I)>	4.7	1.8
Completeness [%]	95.8	98
Redundancy	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6	20 *	ammonium acetate, citrate buffer, MPD, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	0.2 (M)
2	1	reservoir	citrate	0.1 (M)	pH5.6
3	1	reservoir	MPD	30 (%)