1JI5
Dlp-1 from bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.224, 89.224, 210.165 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HP-NAP model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 18.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 20677 | 3107 * |
| <I/σ(I)> | 4.7 | 1.8 |
| Completeness [%] | 95.8 | 98 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 20 * | ammonium acetate, citrate buffer, MPD, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | MPD | 30 (%) |
