1JGE

HLA-B*2705 bound to nona-peptide m9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-10
Detector	MARRESEARCH
Wavelength(s)	0.964
Spacegroup name	P 21 21 21
Unit cell lengths	51.300, 83.200, 110.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 * - 2.100
R-factor	0.1945
Rwork	0.192
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hsa
RMSD bond length	0.014
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.110
High resolution limit [Å]	2.070	2.070
Rmerge	0.049	0.179 *
Number of reflections	26964 *	1931 *
<I/σ(I)>	30.7	6.5
Completeness [%]	91.3 *	67.1 *
Redundancy	6	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	291	used streak-seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.5
3	1	drop		150 (mM)
4	1	reservoir	PEG8000	28 (%)
5	1	reservoir	Tris	100 (mM)	pH8.0