1JFQ

ANTIGEN-BINDING FRAGMENT OF THE MURINE ANTI-PHENYLARSONATE ANTIBODY 36-71, "FAB 36-71"

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	67.130, 73.050, 46.780
Unit cell angles	90.00, 104.48, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.196
Rwork	0.196
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fab
RMSD bond length	0.013
RMSD bond angle	28.887
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	28861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	298	Rose, D.R., (1990) Proc.Natl.Acad.Sci.USA, 87, 338. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG8000	14.9 (%(w/w))
2	1	drop	protein	4.0 (mg/ml)
3	1	drop	phosphate	50 (mM)
4	1	drop		0.5 (%(w/w))
5	1	reservoir	PEG8000	8.5 (%)
6	1	reservoir	potassium phosphate	50 (mM)	pH7.5
7	1	reservoir		0.5 (%)