1JFM
CRYSTAL STRUCTURE OF MURINE NK CELL LIGAND RAE-1 BETA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 138.415, 138.415, 337.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.850 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27900 |
| Structure solution method | MIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.079 | 0.460 |
| Number of reflections | 38675 * | |
| <I/σ(I)> | 37.5 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 * | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 291 | 30% PEG400 + 375mM (NH4)2SO4, pH 4.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | PIPES | 25 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | 0.02 (%) | pH7.0 | |
| 5 | 1 | reservoir | PEG400 | 30 (%(w/w)) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.375 (M) | |
| 7 | 1 | reservoir | acetate | 100 (mM) | pH4.5 |
