1JFD

STRUCTURE OF INORGANIC PYROPHOSPHATASE

Experimental procedure

Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1991-04
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	110.400, 110.400, 154.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.192 *
Rwork	0.192
R-free	0.26600
RMSD bond length	0.009
RMSD bond angle	0.037
Data reduction software	DENZO
Refinement software	REFMAC

Data quality characteristics

	Overall
High resolution limit [Å]	2.196 *
Rmerge	0.077
Total number of observations	153537 *
Number of reflections	18662
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.1 (M)
2	1	drop	ammonium sulfate	35 (%)
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	ammonium sulfate	45 (%)