1JF4
Crystal Structure Of Component IV Glycera Dibranchiata Monomeric Hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-07 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.940, 33.000, 40.760 |
| Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 * |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.24100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 2.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 30.000 |
| High resolution limit [Å] | 1.800 * | 1.400 |
| Rmerge | 0.049 | 36.000 * |
| Number of reflections | 10227 * | |
| Completeness [%] | 89.0 * | 90 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 277 | PEG 4000, sodium acetate, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 15 (%) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 0.1 (M) | pH4.6 |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
