1JEJ

T4 phage apo BGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-09
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 41
Unit cell lengths	59.369, 59.369, 122.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.197 *
Rwork	0.197
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qkj
RMSD bond length	0.007
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.044	0.223
Total number of observations	74099 *
Number of reflections	14639
<I/σ(I)>	16.5
Completeness [%]	100.0	100
Redundancy	5.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	PEG 10000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG10000	10 (%(w/v))
2	1	drop	Tris-HCl	50 (mM)	pH7.5
3	1	reservoir	PEG10000	20 (%)