1JEH

CRYSTAL STRUCTURE OF YEAST E3, LIPOAMIDE DEHYDROGENASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	FUJI
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	97.100, 158.700, 67.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.202
Rwork	0.202
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3grs
RMSD bond length	0.009
RMSD bond angle	0.763 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MERLOT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.680
High resolution limit [Å]	2.400	2.400
Rmerge	0.067
Total number of observations	97677 *
Number of reflections	33372
Completeness [%]	80.2	42.7
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7	298	Toyoda, T., (1997) J.Biochem.(Tokyo), 121, 1. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-20 (mg/ml)
2	1	1	potassium phosphate	25 (mM)
3	1	2	PEG6000	5 (%(w/v))	with water