1JEA
ALTERED TOPOLOGY AND FLEXIBILITY IN ENGINEERED SUBTILISIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.400, 61.350, 75.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.149 |
| RMSD bond length | 0.013 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 68.6 | 58.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 3 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 25 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 14.5 (%(w/v)sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 29 (%(w/v)sat) |
