1JE8
Two-Component response regulator NarL/DNA Complex: DNA Bending Found in a High Affinity Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2000-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.257, 52.741, 83.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 * - 2.120 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DNA-Binding Domain of 1RNL bound to DNA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | GLRF |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 * | 2.130 |
| Rmerge | 0.048 * | 0.194 * |
| Number of reflections | 69333 | |
| <I/σ(I)> | 19.1 | 5.2 |
| Completeness [%] | 94.0 | 73.3 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | ammonium sulfate, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | TRIS |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NarL | 3.5 (mg/ml) | |
| 10 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 2 | 1 | drop | DNA | 1.3 (mg/ml) | |
| 3 | 1 | drop | 0.04 (M) | ||
| 4 | 1 | drop | glycerol | 0.1 (%(v/v)) | |
| 5 | 1 | drop | 0.1 (mM) | ||
| 6 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 7 | 1 | drop | ammonium sulfate | 0.7 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 9 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
