1JDW
CRYSTAL STRUCTURE AND MECHANISM OF L-ARGININE: GLYCINE AMIDINOTRANSFERASE: A MITOCHONDRIAL ENZYME INVOLVED IN CREATINE BIOSYNTHESIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.600, 83.600, 200.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23100 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.720 * |
| Data reduction software | MOSFLM (V. 5.23) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | |
| Total number of observations | 195790 * | |
| Number of reflections | 51924 | |
| Completeness [%] | 91.4 | 67.4 * |
| Redundancy | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 22 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.3-5 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 2 (%) | |
| 3 | 1 | drop | HEPES | 27 (mM) | |
| 4 | 1 | drop | beta-ME | 1.3 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 3 (%) | |
| 6 | 1 | reservoir | HEPES | 40 (mM) | |
| 7 | 1 | reservoir | beta-ME | 2 (mM) |
