1JDH
CRYSTAL STRUCTURE OF BETA-CATENIN AND HTCF-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS |
| Synchrotron site | APS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.770, 76.033, 159.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.202 * |
| Rwork | 0.203 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 47949 | |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 98.8 | |
| Redundancy | 9.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 2.5 (%(w/v)) | |
| 6 | 1 | reservoir | phosphate-citrate | 44 (mM) | pH4.2 |
| 7 | 1 | reservoir | dithiothreitol | 10 (mM) |
