1JD3
Chorismate lyase G90A mutant with bound product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.246, 64.773, 72.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.030 |
| Rwork | 0.183 |
| R-free | 0.25300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1g81.pdb |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.500 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 9861 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 94.0 | 83 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | PEG 4K, hepes, isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
