1JCV

REDUCED BRIDGE-BROKEN YEAST CU/ZN SUPEROXIDE DISMUTASE LOW TEMPERATURE (-180C) STRUCTURE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1993-05-12
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	118.390, 118.390, 73.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.550
R-free	0.22900 *
RMSD bond length	0.011
RMSD bond angle	24.900 *
Data reduction software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	SHELXL93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.000 *
High resolution limit [Å]	1.550 *	1.550 *
Rmerge	0.092 *
Total number of observations	88085 *
Number of reflections	26394 *
Completeness [%]	86.7 *	54.6 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	glcerol	33 (%)
2	1	drop	MPD	67 (%)
3	1	reservoir	ammonium sulfate	2.0-2.3 (M)
4	1	reservoir	Tris-HCl	50 (mM)
5	1	reservoir		50 (mM)
6	1	reservoir		0.001 (%(w/v))