1JCG
MREB FROM THERMOTOGA MARITIMA, AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.100, 51.100, 292.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.2056 |
| Rwork | 0.206 |
| R-free | 0.27640 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1cjf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.098 | 0.219 |
| Number of reflections | 8166 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 97.7 | 99.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 10.5 * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | CAPS | 0.1 (M) | |
| 4 | 1 | drop | protein | 8 (mg/ml) |
