1JCD
Crystal Structure of a Novel Alanine-Zipper Trimer at 1.3 A Resolution, I6A,L9A,V13A,L16A,V20A,L23A,V27A,M30A,V34A,L48A,M51A mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-05-30 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 21.842, 26.935, 45.805 |
| Unit cell angles | 88.05, 84.95, 87.56 |
Refinement procedure
| Resolution | 50.000 * - 1.300 |
| Rwork | 0.149 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jcc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 * |
| Data reduction software | MADNESS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.047 | 0.106 |
| Total number of observations | 153880 * | |
| Number of reflections | 23835 | |
| <I/σ(I)> | 14.4 | 10.8 |
| Completeness [%] | 93.6 | 83.3 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 * | 4 * | Sodium acetate, sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oxidized glutathione | 1 (mM) | |
| 2 | 1 | drop | glutathione | 1 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 0.1 (M) | pH8.7 |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | protein | 40 (mg/ml) | |
| 7 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.0 |
| 8 | 1 | reservoir | sodium citrate | 1.3 (M) |
