1JC9

TACHYLECTIN 5A FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	2000-08-21
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	I 4
Unit cell lengths	108.934, 108.934, 64.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.250 - 2.010
R-factor	0.183 *
Rwork	0.183
R-free	0.19800
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.250	2.130
High resolution limit [Å]	2.010	2.010
Rmerge	0.107	0.344
Number of reflections	48257
<I/σ(I)>	4.3
Completeness [%]	98.1	94.6
Redundancy	6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	PEG 8000, HEPES, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	PEG8000	10 (%)
4	1	reservoir	ethylene glycol	8 (%)	pH7.0