1JC9
TACHYLECTIN 5A FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 |
| Unit cell lengths | 108.934, 108.934, 64.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.250 - 2.010 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| R-free | 0.19800 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.250 | 2.130 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.107 | 0.344 |
| Number of reflections | 48257 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 98.1 | 94.6 |
| Redundancy | 6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | PEG 8000, HEPES, Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | |
| 3 | 1 | reservoir | PEG8000 | 10 (%) | |
| 4 | 1 | reservoir | ethylene glycol | 8 (%) | pH7.0 |
