1JC1

CRYSTAL STRUCTURE ANALYSIS OF A REDOX-SENSITIVE GREEN FLUORESCENT PROTEIN VARIANT IN A OXIDIZED FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-11-25
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.08
Spacegroup name	P 21 21 2
Unit cell lengths	186.838, 67.606, 56.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.900
Rwork	0.229
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.012 *
RMSD bond angle	2.100 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.058	0.247 *
Total number of observations	203865 *
Number of reflections	56854
<I/σ(I)>	7.2	2.3
Completeness [%]	99.8	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	295	PEG 4000, Lithium Sulfate, Tris, Copper (II) Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	20 (mM)	pH7.9
3	1	reservoir	Tris	0.1 (M)	pH8.5
4	1	reservoir		0.07-0.1 (M)
5	1	reservoir	PEG4000	17-22 (%)
6	1	reservoir	dithiothreitol	5 (mM)