1JBZ
CRYSTAL STRUCTURE ANALYSIS OF A DUAL-WAVELENGTH EMISSION GREEN FLUORESCENT PROTEIN VARIANT AT HIGH PH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.200, 62.374, 68.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.500 |
| Rwork | 0.177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 0.319 |
| Total number of observations | 120478 * | |
| Number of reflections | 34536 | |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 96.2 | 93.6 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 295 | PEG 4000, Tris, Magnesium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 48 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.9 |
| 3 | 1 | reservoir | Tris | 50 (mM) | pH9.0 |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 22 (%) |
