1JBW
FPGS-AMPPCP-folate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.100, 45.600, 82.700 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.850 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.088 | 0.519 |
| Total number of observations | 268516 * | |
| Number of reflections | 33586 | |
| <I/σ(I)> | 19.2 | 3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.4 | 291 | 26% PEG1500, 120mM KCl, 20mM Hepes, 2mM 5,10-methylenetetrahydrofolate, 20mM MgCl2, 10mM AMPPCP, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | AMPPCP | 10 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | mTHF | 2 (mM) | |
| 5 | 1 | drop | 120 (mM) | ||
| 6 | 1 | drop | HEPES | 20 (mM) | |
| 7 | 1 | reservoir | PEG1500 | 24-26 (%(w/v)) |
