1JBV
FPGS-AMPPCP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.100, 45.000, 84.300 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.950 |
| R-factor | 0.194 * |
| Rwork | 0.194 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.295 |
| Total number of observations | 195787 * | |
| Number of reflections | 28314 | |
| <I/σ(I)> | 23.4 | 3.2 |
| Completeness [%] | 94.0 | 86.1 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 * | Sun, X., (1998) Proc. Natl. Acad. Sci. USA, 95, 6647. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | acetate | 40 (mM) | |
| 2 | 1 | reservoir | MgATP | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 4 (%) | |
| 4 | 1 | drop | FPGS | 5 (mg/ml) | |
| 5 | 1 | drop | MgATP | 10 (mM) |
