1JBK
Crystal Structure of the First Nucelotide Binding Domain of ClpB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786, 0.9784, 0.9632, 0.9941 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.488, 65.686, 79.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.22 * |
| Rwork | 0.222 |
| R-free | 0.24600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.301 * |
| Data reduction software | SCALE |
| Data scaling software | SCALEPACK |
| Phasing software | MADSYS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.142 |
| Number of reflections | 19159 | |
| <I/σ(I)> | 32.6 | 11.9 |
| Completeness [%] | 99.4 * | 96.3 |
| Redundancy | 4.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Li, J., (2001) Acta Crystallogr, D57, 909. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Li, J., (2001) Acta Crystallogr, D57, 909. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | Tris | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | ammonium sulfate | 2.25 (M) | |
| 7 | 1 | reservoir | 10 (mM) | ||
| 8 | 1 | reservoir | adenylylimidodiphosphate | 5 (mM) |
