1JB5
CRYSTAL STRUCTURE OF NTF2 M118E MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.680, 78.210, 41.430 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.098 | 0.272 |
| Total number of observations | 73003 * | |
| Number of reflections | 9197 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | PEG, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 26 (%) |
