1JB0
Crystal Structure of Photosystem I: a Photosynthetic Reaction Center and Core Antenna System from Cyanobacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 63 |
| Unit cell lengths | 281.000, 281.000, 165.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.21700 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 19.610 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.248 |
| Number of reflections | 246961 | |
| <I/σ(I)> | 14 | 2.9 |
| Completeness [%] | 97.1 | 88.9 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.4 | 277 | Fromme, P., (1998) Biochim. Biophys. Acta, 1365, 175. * |
| 1 | Batch method * | 6.4 | 277 | Fromme, P., (1998) Biochim. Biophys. Acta, 1365, 175. * |
| 1 | Batch method * | 6.4 | 277 | Fromme, P., (1998) Biochim. Biophys. Acta, 1365, 175. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 6 (mM) | ||
| 2 | 1 | 1 | chlorophyll | 1 (mM) |
