1JAW

AMINOPEPTIDASE P FROM E. COLI LOW PH FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-08
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	139.710, 139.710, 230.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.182
Rwork	0.182
R-free	0.22700
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.700 *	2.000
Rmerge	0.093 *	0.290 *
Total number of observations	344400 *
Number of reflections	26338 *
<I/σ(I)>	18	4.5
Completeness [%]	83.1 *	67 *
Redundancy	19.2	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3		drop contained 1:1 mixture of protein and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.7 (mg/ml)
2	1	drop		5 (mM)
3	1	reservoir	PEG4000	30-33 (%)
4	1	reservoir	Tris-HCl	100 (mM)