1JAT
Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9115 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.158, 63.797, 53.152 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.600 |
| R-factor | 0.203 * |
| Rwork | 0.203 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.359 |
| Total number of observations | 185078 * | |
| Number of reflections | 36093 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 1000, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | drop | protein | 10 (mg/ml) | |
| 7 | 1 | reservoir | PEG1000 | 35 (%) | |
| 8 | 1 | reservoir | Tris | 50 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 2 (mM) |
