1J9B
ARSENATE REDUCTASE+0.4M ARSENITE FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-01-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.613, 86.613, 116.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.260 |
| R-factor | 0.128 * |
| Rwork | 0.134 |
| R-free | 0.16600 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | X-GEN |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.330 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.059 | 0.684 |
| Total number of observations | 363771 * | |
| Number of reflections | 51735 * | |
| <I/σ(I)> | 23.6 | 1.4 |
| Completeness [%] | 90.1 * | 34.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 5 * | CESIUM SULFATE, ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 1.2 (M) | ||
| 2 | 1 | drop | ammonium acetate | 100 (mM) | pH4.8 |
| 3 | 1 | drop | protein | 30 (mg/ml) |
