1J90

Crystal Structure of Drosophila Deoxyribonucleoside Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-30
Detector	MARRESEARCH
Wavelength(s)	0.933
Spacegroup name	P 21 21 2
Unit cell lengths	119.456, 60.749, 67.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.560
R-factor	0.238 *
Rwork	0.238
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.260 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	2.720
High resolution limit [Å]	2.560	2.500 *
Rmerge	0.076	0.482
Number of reflections	17594
<I/σ(I)>	8.5	2.3
Completeness [%]	99.9	99.9
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	287	ammonium sulfate, mPEG5000, PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	0.1 (M)	pH6.5
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	PEG5000	20 (%(w/v))
4	1	reservoir	PEG400	8-10 (%(v/v))
5	1	drop	protein	10 (mg/ml)
6	1	drop	deoxycytidine	10 (mM)