1J8G
X-ray Analysis of a RNA Tetraplex r(uggggu)4 at Ultra-High Resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 36.154, 36.154, 74.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.610 |
| R-factor | 0.1032 * |
| Rwork | 0.103 |
| R-free | 0.11170 * |
| Structure solution method | MAD |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.620 |
| High resolution limit [Å] | 0.610 | 0.610 |
| Rmerge | 0.066 | 0.339 |
| Number of reflections | 207845 | |
| <I/σ(I)> | 26 | |
| Completeness [%] | 92.9 | 61.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 * | 20mM Sodium Cacodylate (pH 7.0), 12mM spermine tetrachloride, 20mM MgCl2, 80mM SrCl2, 40mM CaCl2, 35% MPD, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Cacodylate | ||
| 2 | 1 | 1 | spermine tetrachloride | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | SrCl2 | ||
| 5 | 1 | 1 | CaCl2 | ||
| 6 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | spermine chloride | 12.0 (mM) | |
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | 40 (mM) | ||
| 6 | 1 | drop | 20 (mM) | ||
| 7 | 1 | drop | MPD | 10 (%(v/v)) | |
| 8 | 1 | reservoir | MPD | 35 (%) |
