1J88
HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), TETRAGONAL CRYSTAL FORM 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1996-05-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.914 |
| Spacegroup name | P 43 |
| Unit cell lengths | 145.080, 145.080, 62.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.690 - 3.200 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f2q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.105 | 0.363 |
| Total number of observations | 159490 * | |
| Number of reflections | 21357 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 97.5 | 87.2 |
| Redundancy | 7.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 23 * | Garman, S.C., (2000) Nature, 406, 259. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.4-1.6 (M) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) |
