1J88

HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), TETRAGONAL CRYSTAL FORM 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	110
Detector technology	CCD
Collection date	1996-05-21
Detector	CUSTOM-MADE
Wavelength(s)	0.914
Spacegroup name	P 43
Unit cell lengths	145.080, 145.080, 62.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.690 - 3.200
R-factor	0.262
Rwork	0.262
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f2q
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.105	0.363
Total number of observations	159490 *
Number of reflections	21357
<I/σ(I)>	20
Completeness [%]	97.5	87.2
Redundancy	7.5	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	23 *	Garman, S.C., (2000) Nature, 406, 259. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.4-1.6 (M)
2	1	reservoir	Tris	100 (mM)
3	1	drop	protein	10 (mg/ml)