1J87

HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), HEXAGONAL CRYSTAL FORM 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	110
Detector technology	CCD
Collection date	1997-11-15
Detector	MARRESEARCH
Wavelength(s)	1.004
Spacegroup name	P 61 2 2
Unit cell lengths	58.680, 58.680, 229.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.230 - 3.200
R-factor	0.264
Rwork	0.264
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f2q
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.103	0.516
Total number of observations	32661 *
Number of reflections	4076
<I/σ(I)>	18.3
Completeness [%]	93.2 *	95.4
Redundancy	8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	23 *	Garman, S.C., (2000) Nature, 406, 259. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.4-1.6 (M)
2	1	reservoir	Tris	100 (mM)
3	1	drop	protein	10 (mg/ml)