1J86
HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), MONOCLINIC CRYSTAL FORM 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1997-08-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.900, 73.790, 79.400 |
| Unit cell angles | 90.00, 117.74, 90.00 |
Refinement procedure
| Resolution | 22.650 - 3.200 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f2q |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.097 | 0.436 |
| Total number of observations | 47238 * | |
| Number of reflections | 11640 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 23. * | Garman, S.C., (2000) Nature, 406, 259. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.4-1.6 (M) | |
| 4 | 1 | reservoir | CHAPS | 8 (mM) |
