1J7V
HUMAN IL-10 / IL-10R1 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 46.230, 46.230, 307.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.231 * |
| Rwork | 0.231 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.122 |
| Total number of observations | 24449 * | |
| Number of reflections | 8189 | |
| <I/σ(I)> | 9.1 | 5.2 |
| Completeness [%] | 92.8 | 75 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | PEG 6000, MgCl2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | Cymal-6 | 2 (mM) | |
| 5 | 1 | reservoir | ADA | 0.1 (M) | |
| 6 | 1 | reservoir | PEG6000 | 8 (%) | |
| 7 | 1 | reservoir | 0.1 (M) |
