1J7L
Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.710, 91.245, 131.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.470 - 2.200 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.219 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.470 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.213 |
| Total number of observations | 95428 * | |
| Number of reflections | 30902 * | |
| <I/σ(I)> | 13.9 | 4.7 |
| Completeness [%] | 96.4 | 95.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Hon, W.C., (1997) Cell (Cambridge,Mass.), 89, 887. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | PEG8000 | 20-30 (%) |
