1J74
Crystal Structure of Mms2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 42.190, 54.540, 108.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.213 * |
Rwork | 0.213 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7d |
RMSD bond length | 0.005 |
RMSD bond angle | 1.265 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.045 | 0.129 |
Total number of observations | 53554 * | |
Number of reflections | 9973 | |
<I/σ(I)> | 37.1 | 20 |
Completeness [%] | 97.9 | 87.6 |
Redundancy | 5.35 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20-24 * | 16% PEG 6000, 25mM NaCl, 100mM citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | HEPES | 50 (mM) | |
3 | 1 | drop | 75 (mM) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
6 | 1 | reservoir | PEG6000 | 16 (%(w/v)) | |
7 | 1 | reservoir | 25 (mM) | ||
8 | 1 | reservoir | citrate | 100 (mM) |