1J72
Crystal Structure of Mutant Macrophage Capping Protein (Cap G) with Actin-severing Activity in the Ca2+-Free Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97178 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 206.623, 206.623, 56.174 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.960 - 2.500 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.29900 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.530 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.431 |
| Number of reflections | 24873 | |
| Completeness [%] | 98.5 | 85.5 |
| Redundancy | 5.86 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M Tris-HCl, pH 7.0-8.0, 3.5 - 4.0 ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
