1J6W
CRYSTAL STRUCTURE OF HAEMOPHILUS INFLUENZAE LUXS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9641 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 129.669, 129.669, 53.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.184 * |
| Number of reflections | 50722 | |
| <I/σ(I)> | 24.6 | |
| Completeness [%] | 99.6 | 98.6 * |
| Redundancy | 7.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 12 * | PEG MME 5000, MES, BME, NaCl, pH 6.25, VAPOR DIFFUSION, HANGING DROP at 285K, temperature 285.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 21 (%) | |
| 2 | 1 | reservoir | Bis-Tris | 100 (mM) | |
| 3 | 1 | reservoir | beta-mercaptoethanol | 30 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
