1J6V
CRYSTAL STRUCTURE OF D. RADIODURANS LUXS, C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9641 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.190, 70.140, 49.730 |
| Unit cell angles | 90.00, 112.03, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.100 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 * | 0.074 * |
| Number of reflections | 9260 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 98.1 * | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG MME 5000, MES, BME, NaCl, , pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 26 (%) | |
| 2 | 1 | reservoir | MES | 100 (mM) | |
| 3 | 1 | reservoir | beta-mercaptoethanol | 30 (mM) | |
| 4 | 1 | drop | protein | 19 (mg/ml) |
