1J6S

Crystal Structure of an RNA Tetraplex (UGAGGU)4 with A-tetrads, G-tetrads, U-tetrads and G-U octads

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 4 21 2
Unit cell lengths	37.984, 37.984, 82.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
Rwork	0.166
R-free	0.18400
RMSD bond length	0.015
RMSD bond angle	2.000
Data reduction software	DENZO
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.400
Rmerge	0.067 *
Total number of observations	572671 *
Number of reflections	22704 *
Completeness [%]	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	1 (mM)
2	1	drop	sodium cacodylate	20 (mM)	pH6.0
3	1	drop		90 (mM)
4	1	drop		10 (mM)
5	1	drop	spermine tetrahydrochloride	6 (mM)
6	1	drop	MPD	2 (%(v/v))
7	1	reservoir	MPD	15 (%)