1J6R
Crystal structure of Activation (AdoMet binding) domain of Methionine synthase (TM0269) from Thermotoga maritima at 2.2 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91837, 0.979029 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.396, 60.740, 81.574 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 36.220 - 2.300 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | RESOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.220 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 27922 | |
| <I/σ(I)> | 14 | 4.4 |
| Completeness [%] | 90.5 | 93.5 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 2.4 M Ammonium Sulfate; 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 7.00 |
