1J6O
Crystal structure of TatD-related deoxyribonuclease (TM0667) from Thermotoga maritima at 1.8 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.918370, 0.979224, 0.978932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.050, 77.250, 84.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.770 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.860 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 21717 | |
| <I/σ(I)> | 17 | 2.6 |
| Completeness [%] | 98.6 | 87.1 |
| Redundancy | 8.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | 19 % iso-Propanol/19 % PEG 4000; 0.095 M citrate pH 5.6, 5 % Glycerol, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 5.60 |
