1J5Y
Crystal structure of transcriptional regulator (TM1602) from Thermotoga maritima at 2.3 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91162, 0.979105, 0.979445 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 90.957, 90.957, 90.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.677 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 11360 | |
| <I/σ(I)> | 18.6 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.8 M NaH2PO4/0.8 M KH2PO4, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 7.50 |
