1J5X
Crystal structure of Glucosamine-6-phosphate deaminase (TM0813) from Thermotoga maritima at 1.8 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980, 0.93218, 0.97999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.960, 72.960, 128.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.054 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 39495 | |
| <I/σ(I)> | 26.5 | 2.8 |
| Completeness [%] | 99.5 | 95.1 |
| Redundancy | 18.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 40% PEG-400, 0.1 M Imidazole pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 8.00 |
