1J5W

Crystal structure of Glycyl-tRNA synthetase alpha chain (TM0216) from Thermotoga maritima at 1.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.925224, 0.979148, 0.979360
Spacegroup name	P 21 21 21
Unit cell lengths	65.400, 66.200, 142.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.197
Rwork	0.197
R-free	0.25400
Structure solution method	MAD
RMSD bond length	1.777
RMSD bond angle	0.020
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	55037
<I/σ(I)>	15.3	2.2
Completeness [%]	98.2	93.6
Redundancy	6.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	30% (v/v)1,2-propanediol, 0.1 M HEPES pH 7.5, 20% (v/v)PEG-400, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 7.50